neuronal nuclei neun (Proteintech)

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Proteintech neuronal nuclei neun
Neuronal Nuclei Neun, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 709 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/neuronal+nuclei/NeuN+Antibody/pm41577835-131-11-35
Average 96 stars, based on 709 article reviews

neuronal nuclei neun - by Bioz Stars, 2026-09

96/100 stars

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Immunohistochemistry:

Article Title: Genetic Ablation of Sarm1 Mitigates Disease Acceleration after Traumatic Brain Injury in the SOD1 G93A Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.
Article Snippet: Objective: Approximately 20% of familial cases of amyotrophic lateral sclerosis (ALS) are caused by mutations in the gene encoding superoxide dismutase 1 (SOD1).. Epidemiological data have identified traumatic brain injury (TBI) as an exogenous risk factor for ALS; however, the mechanisms by which TBI may worsen SOD1 ALS remain largely undefined.. Methods: We sought to determine whether repetitive TBI (rTBI) accelerates disease onset and progression in the transgenic SOD1 mouse ALS model, and whether loss of the primary regulator of axonal degeneration sterile alpha and TIR motif containing 1 (Sarm1) mitigates the histological and behavioral pathophysiology.

Article Title: Genetic ablation of Sarm1 attenuates expression and mislocalization of phosphorylated TDP-43 after mouse repetitive traumatic brain injury
Article Snippet: .. Immunohistochemistry was performed against pTDP-43 Ser − 409/410 (Proteintech, 1:250, Cat# 22309-1-AP, RRID: AB_11182943), pTau Ser−202/Thr205 (AT8, 1:250, Thermo Fisher Scientific, Cat# MN1020, RRID: AB_223647), neuronal nuclei ( NeuN , 1:200, Proteintech, Cat# 26975-1-AP, RRID: AB_2880708), myelin basic protein ( MBP , 1:200, Santa Cruz Biotechnology, Cat# M3821, RRID: AB_1841021), glial fibrillary acidic protein ( GFAP , 1:250, Agilent, Cat# Z0334, RRID: AB_10013382), and ionized calcium binding adaptor molecule 1 ( Iba-1 , 1:250, Wako, Cat# 019-19741, RRID: AB_839504). .. For chromogenic staining, tissue sections labeled with the primary antibodies (NeuN) were incubated with appropriate biotin-conjugated secondary antibodies followed by avidin-biotin complex (Vector Laboratories) incubation and treatment with diaminobenzidine as directed by the manufacturer.

Article Title: Genetic ablation of Sarm1 attenuates expression and mislocalization of phosphorylated TDP-43 after mouse repetitive traumatic brain injury.
Article Snippet: .. Immunohistochemistry was performed against pTDP-43Ser − 409/410 (Proteintech, 1:250, Cat# 22309-1-AP, RRID: AB_11182943), pTauSer−202/Thr205 (AT8, 1:250, Thermo Fisher Scientific, Cat# MN1020, RRID: AB_223647), neuronal nuclei (NeuN, 1:200, Proteintech, Cat# 26975-1-AP, RRID: AB_2880708), myelin basic protein (MBP, 1:200, Santa Cruz Biotechnology, Cat# M3821, RRID: AB_1841021), glial fibrillary acidic protein (GFAP, 1:250, Agilent, Cat# Z0334, RRID: AB_10013382), and ionized calcium binding adaptor molecule 1 (Iba-1, 1:250, Wako, Cat# 019-19741, RRID: AB_839504). .. For chromogenic staining, tissue sections labeled with the primary antibodies (NeuN) were incubated with appropriate biotin-conjugated secondary antibodies followed by avidin-biotin complex (Vector Laboratories) incubation and treatment with diaminobenzidine as directed by the manufacturer.

Binding Assay:

Article Title: Dexmedetomidine attenuates neuroinflammation-mediated hippocampal neurogenesis impairment in sepsis-associated encephalopathy mice through central α2A-adrenoceptor
Article Snippet: .. Sections were blocked with 8% goat serum for 2 h at room temperature, then incubated overnight at 4°C with primary antibodies against the following: BrdU (1:200; Proteintech), doublecortin (DCX; 1:100; Proteintech), GFAP (1:200; Proteintech), neuronal nuclei (NeuN; 1:100; Proteintech), β-tubulin (1:100; Abcam), ionized calcium binding adaptor molecule-1 (Iba-1; 1:200; Proteintech), α2A-adrenoceptor (1:100; Proteintech), α2Badrenoceptor (1:50; Thermo Fisher Scienti c), α2C-adrenoceptor (1:100; Thermo Fisher Scienti c), nestin (1:200; Abcam), IL-1β (1:100; Proteintech), IL-6 (1:100; Proteintech) and TNF-α (1:50; Abcam). .. The secondary antibody was anti-mouse or anti-rabbit IgG (1:500; Invitrogen) conjugated with Alexa Fluor® 488 or Alexa Fluor® 594.

Double Immunostaining:

Article Title: Transient receptor potential vanilloid 4 blockage attenuates pyroptosis in hippocampus of mice following pilocarpine‑induced status epilepticus.
Article Snippet: .. For double immunostaining, freefloating sections were incubated with primary antibodies against GSDMD (cat. no. AF-4012, 1:100, Affinity Biosciences, Melbourne, Australia), neuronal nuclei (NeuN, cat. no. 26975-1-AP, 1:500, Proteintech Group Inc, Wuhan, China), glial fibrillar acidic protein (GFAP, cat. no. MAB360, 1:500, Millipore, Massachusetts, USA), and ionized calcium-binding adapter molecule 1 (Iba-1, cat. no. Ab178847, 1:500, Abcam, Cambridge, UK). .. The sections were then incubated with either biotin-conjugated goat anti-mouse IgG (cat. no. ab6788, 1:2000) or biotinconjugated rabbit anti-goat IgG (cat. no. ab6740, 1:100) (both from Abcam, Cambridge, UK).

Article Title: Transient receptor potential vanilloid 4 blockage attenuates pyroptosis in hippocampus of mice following pilocarpine‑induced status epilepticus
Article Snippet: .. For double immunostaining, free-floating sections were incubated with primary antibodies against GSDMD (cat. no. AF-4012, 1:100, Affinity Biosciences, Melbourne, Australia), neuronal nuclei (NeuN, cat. no. 26975-1-AP, 1:500, Proteintech Group Inc, Wuhan, China), glial fibrillar acidic protein (GFAP, cat. no. MAB360, 1:500, Millipore, Massachusetts, USA), and ionized calcium-binding adapter molecule 1 (Iba-1, cat. no. Ab178847, 1:500, Abcam, Cambridge, UK). .. The sections were then incubated with either biotin-conjugated goat anti-mouse IgG (cat. no. ab6788, 1:2000) or biotin-conjugated rabbit anti-goat IgG (cat. no. ab6740, 1:100) (both from Abcam, Cambridge, UK).

Incubation:

Article Title: Alpha-tocopherol inhibits ferroptosis and promotes neural function recovery in rats with spinal cord injury via downregulating Alox15.
Article Snippet: After washing with PBS 3 times, slices were blocked and permeabilized by the immunol staining blocking buffer (P0102, Beyotime Biotechnology) for 50 minutes at 37°C. .. Slices were incubated overnight at 4°C with the primary antibodies against Alox15 (rabbit, 1:200, Affinity, Cat# DF13494, RRID: AB_2846513), Ptgs2 (rabbit, 1:200, Affinity, Cat# AF7003, RRID: AB_2835311), Gapdh (mouse, 1:200, Proteintech, Wuhan, China, Cat# 60004–1-Ig, RRID: AB_2107436) and neuronal nuclei (NeuN, mouse, 1:200, Proteintech, Cat# 66836–1-Ig, RRID: AB_2882179). .. After 12 hours, slices were washed by PBS 3 times and then incubated with fluorescence secondary antibody at 37°C for 1 hour: fluorescein isothiocyanate (FITC; rabbit, 1:200, ZSGB-BIO, Beijing, China, Cat# ZF0311, RRID: AB_2571576), tetramethylrhodamine isothiocyanate (TRITC; mouse, 1:200, ZSGB-BIO, Cat# ZF-0313, RRID: AB_2571577).

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